WormBase Tree Display for Picture: WBPicture0000014329

WBPicture0000014329 Description: Fig. 2 Cell-death genes are expressed in B.al/rapaav. a GFP under the control of the egl-1 promoter is expressed brightly in B.al/rapaav in the tailof an early fourth larval stage "wild-type" male of genotype nIs343; him-8. Scale bar: 10 um. b The average intensity of Pegl-1::4xNLS::GFP in thenucleus is higher in the dying B.al/rapaav cell than in the surviving B.al/rapaav homolog in early fourth larval stage animals. The B.al/rapaav fatewas assigned based on partial cytoplasmic refractility and/or nuclear position and distance from the midline of the animal. Average fluorescentintensity within the nucleus was measured for confocal images of B.alapaav, B.arapaav and P12.pa in each animal, and the average backgroundintensity was subtracted from each. A.U., arbitrary units. All genotypes include nIs343, and some also contain : him-5(e1490); : him-8; : nIs349.c-e Proapoptotic genes egl-1 and ced-3 are not highly expressed in the surviving B.al/rapaav homolog (c) but are highly expressed in the dyingB.al/rapaav (d). These two images were taken from the same animal, approximately 1.6 um apart. Anterior, left; dorsal, top. Scale bars: 10 um. Full Z-stack of this animal is available as Additional file 2: Movie 2, and two more examples are available as Additional file 3: Movie 3, Additional file 4: Movie 4. These animals were of the genotype nIs343; him-5(e1490); nIs349. e Quantification of nuclear mRNA transcripts of egl-1 and ced-3 in B.al/rapaav and the B.al/rapaav homolog. Only nuclear transcripts were counted, because we could not unambiguously determine to which cellnon-nuclear transcripts belonged. Condensed chromatin (as visualized by DAPI, which stains DNA and shows the chromatin in a dying cell to beslightly brighter and smaller in volume than that in a living cell), a position to the left or right of P12.pa, or expression of Pegl-1::4xNLS::gfp was each interpreted as indicating the secondary cell fate. All animals were of the genotype nIs343, him-5(e1490), nIs349. *: P < 0.005, **: P < 5 10-4
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  Crop: Crop_picture: WBPicture0000014330
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  Acknowledgment: Template: From: <Journal_URL> <Article_URL>. Copyright <Publication_year> <Publisher_URL>.
    Publication_year: 2016
    Article_URL: DOI id 10.1186/s12915-016-0262-5
    Journal_URL: BMCBiology
    Publisher_URL: BiomedCentralLTD
  Reference: WBPaper00049679