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| WBPicture0000011617 | Description | Figure 1. (A to C) DPY-26 is associated with all condensed mitotic chromosomes in young wild- type XX embryos before the onset of dosage compensation. A wild-type XX embryo (<30-cell stage) was stained with propidium iodide, a nucleic acid-intercalating molecule (red), in (A) and with anti-DPY-26 (green) [as in (4)] in (B). A merged image is shown in (C) (white arrows, condensed mitotic chromosomes in anaphase; yellow arrows, interphase nuclei). Scale bar, 10 μm. (D to F) DPY-26 is localized to the X chromosomes of XX animals that have activated dosage compensation. A wild-type XX embryo (>200-cell stage) was triply stained with the DNA- intercalating molecule diamidophenylindole (DAPI) (blue), with anti-DPY-27 (red) in (D), and with anti-DPY-26 (green) in (E). A merged image is shown in (F). Scale bar, 5 μm. (G to I) Enlargements of the boxed nuclei in (D) through (F), respectively. In this optical section both X chromosomes are visible, and the coincident positions of DPY-26 and DPY-27 on the X chromosomes are apparent. Scale bar, 1 μm. Embryos in (D) through (F) were analyzed by wide- field fluorescence microscopy and deconvolution software (23). Images in (A) through (C) and in all subsequent figures were obtained by confocal microscopy. | |||
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| Name | FigA.jpg | ||||
| Depict | Expr_pattern | Expr1570 | |||
| Cellular_component | GO:0000793 | ||||
| GO:0005634 | |||||
| Acknowledgment | Template | From <Article_URL>. Reprinted with permission from <Publisher_URL>. Readers may view, browse, and/or download material for temporary copying purposes only, provided these uses are for noncommercial personal purposes. Except as provided by law, this material may not be further reproduced, distributed, transmitted, modified, adapted, performed, displayed, published, or sold in whole or in part, without prior written permission from the publisher. | |||
| Publication_year | 1996 | ||||
| Article_URL | DOI | id | 10.1126/science.274.5293.1732 | ||
| Journal_URL | Science | ||||
| Publisher_URL | AAAS | ||||
| Reference | WBPaper00002605 |
