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WBPicture0000010156 | Description | Fig. 3. Expression pattern of GFP::Ce-SLO-2 translational fusion construct and whole-mount immunostaining. (A) Expression of Ce-SLO-2::NLS::GFP in the nerve ring. (B) Lateral view of the midbody region showing expression of Ce-SLO-2::NLS::GFP in the nuclei of the ventral cord. Yellow signals are due to auto-fluorescence in C. elegans. (C) Lateral view of the head using differential interference contrast (Nomarski) microscopy. (D) Same focal plane as (C) showing fluorescence from GFP::Ce-SLO-2 product in the neurite extending from the nerve ring to the amphid end. (E) Expression of GFP::Ce-SLO-2 in the ventral cord through the antero-posterior axis. (F) Posterior view of the worm showing the expression of GFP::Ce-SLO-2 product in the ventral cord terminating in the tail region. (G) Wild-type hermaphrodite worms were stained with pre-immune serum and visualized with FITC-conjugated antibodies. No specific signal or very low background signals were detected. H. Whole-mount immunostaining of larval stage (L2) worms stained with anti-Ce-SLO-2 antibody and visualized as explained in (G). Immuno-reactivity of anti-Ce-SLO-2 is shown to be restricted in neuronal cells. Each scale bar represents 20 um. | |||
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Name | F3.jpg | ||||
Depict | Expr_pattern | Expr524 | |||
Anatomy | WBbt:0003679 | ||||
WBbt:0005829 | |||||
WBbt:0006749 | |||||
Cellular_component | GO:0005634 | ||||
Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
Publication_year | 1999 | ||||
Article_URL | DOI | id | 10.1016/S0378-1119(99)00398-4 | ||
Journal_URL | Gene | ||||
Publisher_URL | Elsevier | ||||
Reference | WBPaper00003795 |