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WBPicture0000010156 | Description | Fig. 3. Expression pattern of GFP::Ce-SLO-2 translational fusion construct and whole-mount immunostaining. (A) Expression of Ce-SLO-2::NLS::GFP in the nerve ring. (B) Lateral view of the midbody region showing expression of Ce-SLO-2::NLS::GFP in the nuclei of the ventral cord. Yellow signals are due to auto-fluorescence in C. elegans. (C) Lateral view of the head using differential interference contrast (Nomarski) microscopy. (D) Same focal plane as (C) showing fluorescence from GFP::Ce-SLO-2 product in the neurite extending from the nerve ring to the amphid end. (E) Expression of GFP::Ce-SLO-2 in the ventral cord through the antero-posterior axis. (F) Posterior view of the worm showing the expression of GFP::Ce-SLO-2 product in the ventral cord terminating in the tail region. (G) Wild-type hermaphrodite worms were stained with pre-immune serum and visualized with FITC-conjugated antibodies. No specific signal or very low background signals were detected. H. Whole-mount immunostaining of larval stage (L2) worms stained with anti-Ce-SLO-2 antibody and visualized as explained in (G). Immuno-reactivity of anti-Ce-SLO-2 is shown to be restricted in neuronal cells. Each scale bar represents 20 um. | |
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Name | F3.jpg | ||
Depict | Expr_pattern | Expr524 | |
Anatomy | WBbt:0003679 | ||
WBbt:0005829 | |||
WBbt:0006749 | |||
Cellular_component | GO:0005634 | ||
Acknowledgment (5) | |||
Reference | WBPaper00003795 |