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| WBPicture0000009747 | Description | Figure 3. unc-75 Functions in Neurons and Localizes to Subnuclear Speckles. (A) Mosaic analysis of unc-75 function. AB lineages give rise to the majority of neurons, while P1 lineages give rise to the germ line (P4), the intestine (E), hypodermis, coelomocytes, and muscle (MS, C, D). An extrachromosomal array was created that contains the rescuing C17D12 cosmid and two GFP reporters that mark the neuronal and muscle lineages (see the Supplemental Experimental Procedures). The arrows in the lineage diagram indicate the point at which the array was lost in mosaic animals. Early array loss at AB leads to no rescue in animals (red arrow), while animals showing losses at P1 were still rescued (green arrow). 'n' refers to the number of mosaic animals of each class that were scored. For simplicity, additional classes of more compound mosaics are not shown.(B-F) The expression pattern of unc-75 reporter gene constructs. Multiple lines showed similar patterns of expression. (B and C) Expression of promoter fusions in embryos at the (B) 300-cell stage and in (C) L1 larvae. (D) High magnification of ventral cord neuronal nuclei reveals that the rescuing UNC-75::GFP protein localizes to subnuclear puncta (green channel, top). UNC-75::GFP puncta do not colocalize with DAPI-stained DNA (lower two panels). (E) Deletion of the predicted nuclear localization signal (Figure 2) leads to loss of UNC-75::GFP subnuclear localization and a diffuse redistribution throughout the nucleo- and cytoplasm, including axons. (F) Subnuclear UNC-75::GFP puncta are dynamic. Images of immobilized animals taken at 10-s intervals in the same focal plane show that UNC-75 speckles are continuously moving/remodeling with the nucleus. See the Supplemental Data for the corresponding movie.(G) Human CELF4/BrunoL4::GFP localizes to subnuclear speckles in transgenic worms. | |||
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| Name | F3.jpg | ||||
| Crop | Crop_picture | WBPicture0000009748 | |||
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| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
| Publication_year | 2003 | ||||
| Article_URL | DOI | id | 10.1016/S0960-9822(03)00532-3 | ||
| Journal_URL | CurrentBiology | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00006029 |
