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| WBPicture0000009731 | Description | Figure 3. Dynamin Localizes to the Newly Formed Furrow Membranes and the Midbody in C. elegans. All embryos are arranged with anterior toward the left.(A-E) Cells at the middle and late stages of mitosis were stained for alpha-Tubulin (green) and Dyn-1 (red) and were observed with a Leica confocal microscope as described previously [36]. ([A], arrow) At the metaphase-anaphase transition, dynamin was observed to localize between the asters. (B) During anaphase, dynamin appeared to localize equatorially, and a population of dynamin appeared on the midzone microtubules. ([C], arrow) During late telophase, dynamin appeared to localize only to the newly formed membrane. ([D], arrow) During early cytokinesis, dynamin localized to the newly formed membrane and along the cleavage furrow. ([E], arrow) As cytokinesis progressed, the dynamin staining became more punctate along the newly ingressed furrow, and the midbody staining was more intense.(F-I') To deplete C. elegans embryos and adults of dynamin, embryos and adults were treated with dyn-1 RNAi and were then stained, as described previously [36], for (F-I) microtubules (as a marker for the stages of mitosis) and (F'-I') dynamin. (G' and I') A marked reduction in dynamin staining as well as a (G and I) disruption of the microtubule cytoskeleton can be seen in the (G and G') early embryo and (I and I') adult gonad treated with dyn-1 RNAi as compared to (F and F' and H and H'; all gonad images are of the same focal plane) untreated control animals.(J and J') In the wild-type C. elegans gonad, dynamin resides just inside the tubulin meshwork. (J) Top and (J') midfocal planes of merged alpha-Tubulin (green) and dynamin (red) staining in the gonad are shown.The scale bars represent 5 μm. | |||
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| Name | F3.jpg | ||||
| Depict | Expr_pattern | Expr2307 | |||
| Cellular_component | GO:0005876 | ||||
| GO:0030496 | |||||
| GO:0032154 | |||||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
| Publication_year | 2002 | ||||
| Article_URL | DOI | id | 10.1016/S0960-9822(02)01390-8 | ||
| Journal_URL | CurrentBiology | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00005664 |
