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WBPicture0000009722DescriptionFigure 3. PAT-4/ILK and bPAT-3 Integrin Colocalize at Muscle Attachments. (A) A wild-type, ~420 min embryo. PAT-4::GFP localizes to body-wall muscle attachments (arrow). The scale bar represents 2 μm.(B) The same embryo as that shown in (A) double stained with monoclonal antibodies MH25, recognizing ²PAT-3 integrin, and MH27, recognizing hypodermal-hypodermal cell junctions (included for developmental staging and orientation purposes).(C) An overlay of (A) and (B). Areas of PAT-4::GFP and integrin colocalization appear yellow.(D-F) Detail of body-wall muscle from a rescued pat-4(st551) adult hermaphrodite coexpressing (D) pat-4::yfp and (E) pat-3::cfp. Dense body (arrows) and M-line (arrowheads) attachment structures are indicated. (F) An overlay of panels (D) and (E). Regions in which betaPAT-3::CFP and PAT-4::YFP colocalize appear white. The scale bar in (D) represents 5 μm.
NameF3.jpg
DepictExpr_patternExpr1888
AnatomyWBbt:0005813
Cellular_componentGO:0031430
GO:0055120
AcknowledgmentTemplateReprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
Publication_year2002
Article_URLDOIid10.1016/S0960-9822(02)00810-2
Journal_URLCurrentBiology
Publisher_URLElsevier
ReferenceWBPaper00005261