WormBase Tree Display for Picture: WBPicture0000009587

WBPicture0000009587 Description: Figure 5. VAB-8 Expression. (A and B) Immunofluorescence photomicrographs of animals carrying vab-8-gfp transgenes.(A) Head of transgenic larvae expressing VAB-8L-GFP from pFWV8LG. VAB-8 is expressed in the AVK (arrowhead). Axons in the head, including axons of the nerve ring neuropil indicated with an arrow, also express VAB-8. Anterior is to the left. Scale bar, 20 μm.(B) Transgenic embryo expressing VAB-8S-GFP from pFW34. VAB-8 (green) is expressed in the CAN cell (arrowhead) as well as many cells in the head and tail. Muscles are stained with vinculin antibodies to outline embryo (red; MH24). Scale bar, 10 μm.(C) Diagram of C. elegans head showing actin-rich musculature, including dorsal and ventral body wall muscle sectors in dark gray and the pharynx in light gray. The region represented in (D) is boxed.(D) Immunofluorescence photomicrograph of the head of a wild-type adult hermaphrodite stained with an anti-VAB-8 antiserum (green) and rhodamine-conjugated phalloidin to detect actin (red). Anterior is to the left. Arrowheads indicate the anterior ends of individual muscle cells. The intense phalloidin staining is pharyngeal muscle actin. Boxed area is enlarged in (E). Scale bar, 10 μm.(F) Confocal image of a wild-type adult hermaphrodite stained with and anti-VAB-8 antiserum (green) and an anti-β-integrin PAT-3 antiserum (red) to detect dense bodies. Scale bar, 5 μm.
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  Depict: Expr_pattern: Expr1433
    Anatomy: WBbt:0005813
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      WBbt:0006823
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  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
    Publication_year: 1998
    Article_URL: DOI id 10.1016/S0896-6273(00)81006-5
    Journal_URL: Neuron
    Publisher_URL: Elsevier
  Reference: WBPaper00003058