WormBase Tree Display for Picture: WBPicture0000009515

WBPicture0000009515 Description: Figure 4. MIX-1 Localizes to All Chromosomes in Every Mitotic Cell All panels are false-color confocal images.(A-C) Wild-type XX embryo costained with PI (A) and anti-phosphorylated histone H3 antibody (B). Merged image in (C). Histone H3 is phosphorylated from prometaphase to telophase, making it an ideal marker for the M phase of the cell cycle (Hendzel et al. 1997). In this embryo, phosphorylated histone H3 is present in five nuclei, four of which are in metaphase (bar-shaped chromosomes) and one in prometaphase.(D) Merged image of a wild-type one-celled XX embryo costained with anti-alpha-tubulin (red) and MIX-1 (green) antibodies, showing that MIX-1 is coincident with the mitotic chromosomes on the metaphase plate.(E) Merged image of a wild-type two-celled XX embryo stained with anti-alpha-tubulin (red) and MIX-1 (green) antibodies. The AB cell (left) is in metaphase, but the P1 cell (right) has not yet initiated mitosis. No MIX-1 staining is evident in the P1 cell, which divides 3 min after AB.(F-H) An (500-cell) embryo costained with anti-phosphorylated histone H3 (F; red) and anti-MIX-1 (G; green) antibodies. Merged image in (H). MIX-1 is localized to all of the chromosomes in mitotic cells but is localized only to X in interphase nuclei (yellow arrow).(I) Merged image of an L1 larvae costained with anti-phosphorylated histone H3 (red) and anti-DPY-26 (green) antibodies. DPY-26 (yellow dots) is only associated with X among the prometaphase mitotic chromosomes.Scale bars, 10 μm in (A-H) and 25 μm in (I).
  Name: F4.jpg
  Depict: Expr_pattern: Expr1443
    Cellular_component: GO:0005694
  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
    Publication_year: 1998
    Article_URL: DOI id 10.1016/S0092-8674(00)80920-4
    Journal_URL: Cell
    Publisher_URL: Elsevier
  Reference: WBPaper00002999