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WBPicture0000008948DescriptionFigure 4. Cellular expression of Ce- Duox1. Animals were immunostained with antibodies to Ce-Duox1 (A-C, E, F, and H, green), myosin A (A and B, red), and MH4 (D, E, G, and H, red), and fluo- rescence was visualized using confocal microscopy. Merged images of Ce-Duox1 and myosin A staining (A and B) show that the Ce-Duox1 is expressed in a layer of cells just outside the muscle bundles. Longitudinal images (C and D) and cross section images (F and G) show the indi- vidual staining patters of Ce-Duox1 and the hypodermal cell marker MH4, respectively. Merged images (E and H) show areas of overlapping staining of Ce-Duox1 and MH4 in yellow, confirm- ing the expression of Ce-Duox1 in the hy- podermal layer of cells. The use of antibody to Ce-Duox1 that had been preincubated with Ce-Duox1(340-355) peptide eliminated green channel anti- body staining (unpublished data). Photos are representative of >100 animals observed.
NameF4.large.jpg
DepictExpr_patternExpr2613
AnatomyWBbt:0005733
Acknowledgment (5)
ReferenceWBPaper00004841