WormBase Tree Display for Expr_pattern: Expr2613
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| Expr2613 | Expression_of | Gene | WBGene00000253 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000253 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| Anatomy_term | WBbt:0005733 | Certain | ||
| Type | Antibody | Rabbit polyclonal antibody against a 16 amino acid peptide corresponding to residues 340-355 of Ce-Duox1. | ||
| Pattern | Ce-Duox1 immunostaining was seen in larval animals in a "string-of-pearls" pattern immediately peripheral to muscle. Ce-Duox1 was also visualized immediately peripheral to muscle bundles in the cross-sectional plane. Ce-Duox1 staining was considerably weaker in adult animals. | |||
| The above pattern is consistent with a localization of Ce-Duox1 in hypodermal cells. To verify this localization, double staining was carried out with antibodies to Ce-Duox1 and to the hypodermal cell protein MH4. Comparison shows that expression of Ce-Duox1 and MH4 occurs in the same cellular pattern, although the staining intensity differed in different regions of the hypodermal cell layer. | ||||
| Picture | WBPicture0000008948 | |||
| Remark | Immunostaining with antibody to Ce-Duox1 that had been preincubated with Ce-Duox1(340355) peptide and antimyosin A antibodies eliminated staining in the green channel, demonstrating antibody specificity. | |||
| bli-3 = F56C11.1 = Ce-Duox1 | ||||
| Reference | WBPaper00004841 | |||
| Antibody_info | WBAntibody00000404 |
