WormBase Tree Display for Picture: WBPicture0000008923

WBPicture0000008923 Description: Figure 2. Distribution of cytoplasmic dynein in early wild-type embryos. Embryos stained with anti-DHC-1 and antitubulin (TUB; high magnification images only) antibodies and counterstained with Hoechst 33258 to reveal DNA. A, B, O, and P are at the same magnification, as are C-N. Merged images, DHC-1, red; TUB, green; and DNA, blue. (A and B) One cell stage embryo during pronuclear migration. DHC-1 is distributed throughout the cytoplasm in a punctate manner, and is enriched at the periphery of the male (A and B, arrowhead) and female (A and B, arrow) pronuclei. DHC-1 is also slightly enriched at the cortex of one cell stage embryos, but this is not rendered in this particular focal plane. Small arrowhead in B points to out-of-focus polar body DNA. (C-F) Prometaphase, one cell stage embryo. DHC-1 is enriched on both sides (C and F, arrows) of congressing chromosomes (D and F, arrowhead). (G-J) Metaphase, one cell stage embryo. DHC-1 is enriched on the spindle on both sides of the metaphase plate. (K-N) Early anaphase, P1 blastomere of two cell stage embryo. DHC-1 is enriched on the spindle between the chromosomes (L and N, arrowheads) and the spindle poles, as well as centrally (K and N, arrow) between the two sets of chromosomes. (O and P) Two cell stage embryo, P1 blastomere (right) is in late anaphase, AB blastomere (left) in late telophase. In P1, DHC-1 is enriched on the spindle (O, arrow) between the chromosomes and the spindle poles and in the central spindle. In AB, DHC-1 is enriched at the periphery of reforming nuclei (O, black arrowhead) as well as in an area above the spindle poles. In addition, DHC-1 is localized throughout the cortex between the AB and P1 blastomeres (O, arrowheads). Small arrowhead in P points to polar body DNA. Bars, 10 um.
  Name: F2.large.jpg
  Depict: Expr_pattern: Expr1196
    Cellular_component: GO:0000922
      GO:0005737
      GO:0005938
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. The user must not use the Work for commercial purposes. If the user shares, alters, transforms, or builds upon the Work, the user may distribute the resulting work only under the same license the user received from <Publisher_URL>.
    Publication_year: 1999
    Article_URL: DOI id 10.1083/jcb.147.1.135
    Journal_URL: TheJournalofCellBiology
    Publisher_URL: TheRockefellerUniversityPress
  Reference: WBPaper00003704