WormBase Tree Display for Expr_pattern: Expr1196
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| Expr1196 | Expression_of | Gene | WBGene00000962 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000962 | ||
| Expression_data | Life_stage | WBls:0000005 | |
| GO_term | GO:0005737 | ||
| GO:0005938 | |||
| GO:0000922 | |||
| Subcellular_localization | Cytoplasmic dynein was present in a punctate manner throughout the cytoplasm at all stages of the cell cycle. A stronger signal was detected at the periphery of pronuclei in one cell stage embryos and of nuclei in later stage embryos. Cytoplasmic dynein was present at the cell cortex; especially apparent at boundaries between cells, for instance, between the AB and P1 blastomeres of the two cell stage embryo. The distribution of cytoplasmic dynein changed as cells progressed through mitosis. During prometaphase, cytoplasmic dynein accumulated along both sides of prometaphase chromosomes. During early anaphase, strong spindle signal was still detected, both between segregating chromosomes and spindle poles, as well as centrally, between the two sets of chromosomes. A similar staining pattern persisted throughout anaphase. At telophase, cytoplasmic dynein was enriched in two areas of the cytoplasm adjacent to the spindle poles. A strong signal was detected at the periphery of reforming nuclei. | ||
| Type | Antibody | AntiDHC-1 antibodies to determine the subcellular distribution of cytoplasmic dynein in early wild-type embryos by immunofluorescence microscopy. | |
| Picture | WBPicture0000008923 | ||
| Remark | A subcellular distribution analogous to the one reported here was observed in C. elegans embryos using polyclonal antibodies raised against purified dynein heavy chain protein. | ||
| Reference | WBPaper00003704 | ||
| Antibody_info | WBAntibody00000242 |
