WormBase Tree Display for Picture: WBPicture0000008693
expand all nodes | collapse all nodes | view schema
| WBPicture0000008693 | Description | Figure 2. Expression pattern of crt-1 in C. elegans. (A) Northern blot analysis with 32P-labeled crt-1 probe detects a 1.4-kb band at all developmental stages. Eggs (E); larval stages (L2-L4); adults (A). (B-E) In situ localization of crt-1 RNA at different developmental stages. With the use of an antisense probe, robust signals are observed in a two-cell stage embryo and in a four-cell stage embryo. Restricted signals along the midline are observed in intestinal precursor cells, arrowhead indicates anterior part of the embryo. (C) DAPI-stained embryos shown in B to visualize the nuclei. (D) Strong signal is observed along the intestine of a larva and in the pharyngeal bulb (E, arrowheads). (F-I) Immunofluorescence localization of CRT-1 in wild-type worms with the use of anti-CRT-1 antibodies. Staining is observed in the cytoplasm of early embryo stage (F), along the intestinal wall of a larva (G), and in the terminal bulb region of the pharynx (arrows) and some excretory cells (arrowhead) in the head (H). (I) DAPI-stained worm shown in H. Bar, 20 um. (J-L) Immunogold electron micrograph showing the subcellular localization of CRT-1. (J) Transverse section of the intestine showing clustered signals of CRT-1 as gold particles around the intestinal vacuoles (arrows) in wild-type worms. (K) Transverse section of the body-wall muscle structure showing CRT-1 signals (arrow) at the cytoplasmic face of the muscle cells in wild-type animals. (L) Section through the pharynx shows signals in the cytoplasmic regions between muscular structure. Bar, 0.5 um. | |||
|---|---|---|---|---|---|
| Name | F2.large.jpg | ||||
| Crop | Crop_picture | WBPicture0000008694 | |||
| WBPicture0000008695 | |||||
| WBPicture0000008696 | |||||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article <Article_URL>. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 2001 | ||||
| Article_URL | DOI | id | 10.1091/mbc.12.9.2835 | ||
| Journal_URL | MolecularBiologyoftheCell | ||||
| Publisher_URL | TheAmericanSocietyForCellBiology | ||||
| Reference | WBPaper00004858 |
