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WBPicture0000008644 | Description | Figure 6. Expression of heteromeric kinesin promoter::GFP constructs in the C. elegans nervous system. Heritable lines of worms carrying extrachromosomal arrays of CeKRP95 promoter::GFP, osm-3 promoter::GFP, or CeKAP promoter::GFP fusion constructs were created and imaged by laser scanning confocal microscopy. Genomic sequences upstream from the CeKRP95, Osm-3, and CeKAP genes were fused to a GFP reporter, and the constructs were singularly coinjected into hermaphrodite gonads of N2 wild-type worms with the dominant rol-6 marker. Heritable lines of worms displaying the roller phenotype were isolated and analyzed for GFP expression. Shown are the head (left) and tail (right) regions of CeKRP95 promoter-GFP transgenics (A and B), osm-3 promoter::GFP transgenics (C and D), and CeKAP promoter::GFP transgenics (E and F). CB, cell body; D, dendrite; NC, nerve cords and associated commissures. (B, D, and F) GFP expression in the phasmid chemosensory neurons of the tail; (A, C, and E) GFP expression in the cell bodies and dendrites of the amphid and inner labial chemosensory neurons in the head. A and B also indicate widespread neuronal expression in CeKRP95 promoter::GFP worms, with GFP being expressed in the nerve ring and the dorsal and ventral nerve cords and associated commissures. | |||
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Crop | Crop_picture | WBPicture0000008645 | |||
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Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article <Article_URL>. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
Publication_year | 1999 | ||||
Article_URL | DOI | id | 10.1091/mbc.10.2.345 | ||
Journal_URL | MolecularBiologyoftheCell | ||||
Publisher_URL | TheAmericanSocietyForCellBiology | ||||
Reference | WBPaper00003466 |