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| WBPicture0000008405 | Description | Figure 5. Expression of cpb-1 mRNA and protein. (A) Northern blot showing the tissue distribution ofcpb-1 mRNA. Total RNA (15 μg) was isolated from adults of the indicated strains and was probed with a cpb-1-specific probe. The rpl-12 gene encoding ribosomal protein L12 is included as a loading control. The position of cpb-1 mRNA is indicated. (B) Exon diagram of the cpb-1 transcript. The RRMs and zinc fingers are indicated by black and hatched boxes, respectively. Gray boxes represent the coding region; white boxes represent 5' and 3' UTR sequences. Bracket indicates the region of CPB-1 used to raise antibodies and also used as bait in the two-hybrid screen. (C) CPB-1 protein expression in the hermaphrodite germ line. Gonads were extruded and stained with affinity-purified CPB-1 antibodies (red) and the sperm-specific SP56 antibody (green). Panels 1 and 2 are L4 gonads. Panels 3 and 4 are adult gonads. (Left) Wild type; (right)cpb-1(RNAi) controls. White arrowheads indicate the proximal end of the gonad. Inset in panel 1 shows an enlargement of the CPB-1-stained region. | |||
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| Name | F2.large.jpg | ||||
| Depict | Expr_pattern | Expr1052 | |||
| Anatomy | WBbt:0005784 | ||||
| Acknowledgment | Template | WormBase wishes to thank <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 2000 | ||||
| Article_URL | DOI | id | 10.1101/gad.831700 | ||
| Journal_URL | Genes&Development | ||||
| Publisher_URL | ColdSpringHarborLabPress | ||||
| Reference | WBPaper00004396 |
