WormBase Tree Display for Expr_pattern: Expr1052
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Expr1052 | Expression_of | Gene | WBGene00000770 | |
---|---|---|---|---|
Reflects_endogenous_expression_of | WBGene00000770 | |||
Expression_data | Life_stage | WBls:0000038 | ||
Anatomy_term | WBbt:0005784 | Certain | ||
GO_term | GO:0005737 | |||
Subcellular_localization | CPB-1 staining was predominantly cytoplasmic, as indicated by a uniform strong signal that is excluded from nuclei. This cytoplasmic staining was specific: It was not detectable in cpb-1(RNAi) L4 hermaphrodites. The same pattern was observed in males. In contrast, a faint nonspecific staining was detected in nuclei throughout the gonad. | |||
Type | Antibody | Affinity-purified polyclonal antibodies against the nonconserved N-terminal 258 amino acids of the protein. Doubly stained extruded gonads of wild-type and cpb-1(RNAi) hermaphrodites with anti-CPB-1 antibodies and the monoclonal SP56 antibody, which recognizes cells undergoing spermatogenesis, beginning at least as early as the primary spermatocyte stage. | ||
Pattern | CPB-1 protein was detected at high levels in the L4 germ line, in a region just distal to the region that stained by SP56. An identical distribution was observed in males. | |||
Picture | WBPicture0000008405 | |||
Remark | CPB-1 protein was not detectable in adult germ lines that have switched to oogenesis. Thus, although cpb-1 mRNA is present in adult hermaphrodites, which are making only oocytes, the protein is not. | |||
Reference | WBPaper00004396 | |||
Antibody_info | WBAntibody00000040 | |||
WBAntibody00000326 |