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WBPicture0000008249 | Description | Figure 1. Expression patterns for NHX-2, a sodium-proton exchanger, and OPT-2, a proton-coupled oligopeptide transporter, using promoter::GFP transgenic reporter strains. GFP reporter expression in transgenic nematodes was driven by genomic DNA fragments containing either 4 kb of promoter alone (panels A-F) or 4 kb of promoter along with the entire open reading frame of the cognate protein, fused in-frame with GFP (panels G-L). The nhx-2 gene promoter (panels A and B) and the opt-2 gene promoter (panels C and D) drove GFP expression in the gut of the worm. The opt-1 gene promoter caused GFP to be expressed primarily in pharyngeal and vulval regions (panels E and F). The NHX-2 (panels G and H) and the OPT-2 (panels I-L) fusion proteins were targeted to the apical membrane of the intestine, which expands prior to contacting the pharynx, as demonstrated in panel L. | |||
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Name | F1.large.jpg | ||||
Crop | Crop_picture | WBPicture0000008250 | |||
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Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL> <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
Publication_year | 2003 | ||||
Article_URL | DOI | id | 10.1074/jbc.M307351200 | ||
Journal_URL | TheJournalofBiologicalChemistry | ||||
Publisher_URL | TheAmericanSocietyForBiochemistryandMolecularBiology | ||||
Reference | WBPaper00006186 |