WormBase Tree Display for Picture: WBPicture0000008249
expand all nodes | collapse all nodes | view schema
WBPicture0000008249 | Description | Figure 1. Expression patterns for NHX-2, a sodium-proton exchanger, and OPT-2, a proton-coupled oligopeptide transporter, using promoter::GFP transgenic reporter strains. GFP reporter expression in transgenic nematodes was driven by genomic DNA fragments containing either 4 kb of promoter alone (panels A-F) or 4 kb of promoter along with the entire open reading frame of the cognate protein, fused in-frame with GFP (panels G-L). The nhx-2 gene promoter (panels A and B) and the opt-2 gene promoter (panels C and D) drove GFP expression in the gut of the worm. The opt-1 gene promoter caused GFP to be expressed primarily in pharyngeal and vulval regions (panels E and F). The NHX-2 (panels G and H) and the OPT-2 (panels I-L) fusion proteins were targeted to the apical membrane of the intestine, which expands prior to contacting the pharynx, as demonstrated in panel L. | |
---|---|---|---|
Name | F1.large.jpg | ||
Crop | Crop_picture | WBPicture0000008250 | |
WBPicture0000008251 | |||
Acknowledgment (5) | |||
Reference | WBPaper00006186 |