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WBPicture0000007485DescriptionFigure 5. UNC-83 is localized to the nuclear envelope. In all panels, anterior is leftwards, and dorsal is upwards. UNC-83 antibody localization is pseudocolored red, and DAPI staining is in blue. In some panels, JAM-1::GFP is used to show adherens junctions in green. (A-C) Preelongation embryos, examples of hyp7 nuclei are marked with arrowheads. (A) unc-83(e1408), a putative null. (B) Wild-type. (C) unc-83(ku18), an allele in which hyp7 nuclear migration is not disrupted. (D-F) Lateral views of sections through a single wild-type embryo at the onset of elongation (bean stage). (D) A section near the top with P-cell nuclei marked by arrowheads. (E) A section 1 um lower showing hyp7 nuclei marked with arrowheads. (F) A section 4 um lower showing UNC-83 staining in intestinal cells marked with arrowheads. (G-I) Wildtype worms. (G) A lateral view of a hermaphrodite late in the first larval stage showing UNC-83 in marked P cells. The arrow marks an intestinal cell in the same plane. (H) The tail of an adult worm. UNC-83 stained many hypodermal cells, examples are marked with arrowheads, a large intestinal cell (arrow) and other unidentified cells. (I) The head of an adult worm showing UNC-83 staining in hypodermal (examples marked with arrowheads) and other unidentified cells. Scale bars: in F, 10 um for A-F; in G, 9 um for G; in I, 25 um for H,I.
NameF5.large.jpg
DepictExpr_patternExpr1789
AnatomyWBbt:0005734
WBbt:0005772
Cellular_componentGO:0005635
AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year2001
Journal_URLDevelopment
Publisher_URLTheCompanyofBiologists
ReferenceWBPaper00005053