WormBase Tree Display for Expr_pattern: Expr1789
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| Expr1789 | Expression_of | Gene | WBGene00006815 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006815 | ||
| Expression_data | Life_stage | WBls:0000024 | |
| WBls:0000003 | |||
| WBls:0000038 | |||
| WBls:0000027 | |||
| WBls:0000035 | |||
| WBls:0000041 | |||
| Anatomy_term (16) | |||
| GO_term | GO:0005635 | ||
| Subcellular_localization | Antibodies against UNC-83 stained the nuclear envelopes of migratory nuclei and several additional nuclei. | ||
| Type | Antibody | Mouse monoclonal antibodies against a maltose-binding protein fusion protein containing most of UNC-83 (from the initiator methionine of UNC-83C to just before the carboxyl predicted transmembrane domain). | |
| Pattern | UNC-83 was not detected at the nuclear envelope of migrating pronuclei. UNC-83 was first detected at the nuclear envelopes of migrating embryonic hyp7 nuclei. Later, at the bean stage of embryonic development, UNC-83 was localized to the nuclear envelopes of hyp7 cells, P cells and intestinal cells. By the comma stage, UNC-83 was detected in these cells as well as in several additional cells in the pharynx. In late stages of wild-type embryos, and in larvae and adults, UNC-83 was detected on nuclei in a wide variety of cells, including several cells around the pharynx and in the uterus. | ||
| Picture | WBPicture0000007485 | ||
| WBPicture0000007486 | |||
| Remark | The immunostaining patterns described here were not present in putative null unc-83 mutants and thus are specific for the UNC-83 protein. | ||
| UNC-83 co-localized with two other proteins of the nuclear envelope, UNC-84 and lamin. UNC-83 has a more punctate staining pattern than Lamin. It also appears that UNC-83 is more punctate that UNC-84. This may simply be due to the fact that UNC-84 was detected as a GFP fusion protein that is likely to be overexpressed. | |||
| unc-83(ku18) mutants, which have nuclear migration defects in the P cells and the intestinal cells but not in hyp7 precursors, showed immunostaining for UNC-83 in the hyp7 nuclei but not in the P cell nuclei or intestinal nuclei. | |||
| Reference | WBPaper00005053 | ||
| Antibody_info | WBAntibody00000447 |
