WormBase Tree Display for Expression_cluster: WBPaper00045729:gld-4(RNAi)_upregulated
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| WBPaper00045729:gld-4(RNAi)_upregulated | Description | Genes that showed significantly increased expression in gld-4(RNAi) comparing with control RNAi, according to RNAseq. | |
|---|---|---|---|
| Species | Caenorhabditis elegans | ||
| Algorithm | Using the DESeq package (R 3.0.2, DESeq version 1.14.0), the count data were normalized by the size factor to estimate the effective library size. After calculating the gene dispersion across all samples, the comparison of two different conditions resulted in a list of differentially expressed genes. Genes with a normalized read count smaller than 100 were ignored in the final analysis. A pre-filtering step was used to calculate the number of genes showing a high probability of being differentially regulated. In this step, genes with a probability (unadjusted P-value) above 0.003 were excluded from the differential expression analysis. Genes with a fold-change higher or equal to two, as well as an adjusted P-value of <= 0.05 was then defined as differentially expressed. The P-values are being adjusted to the multiple testing hypotheses to reduce the false discovery rate (FDR). | ||
| Reference | WBPaper00045729 | ||
| Based_on_WB_Release | 220 | ||
| Gene (22) | |||
| Attribute_of | RNASeq | ||
| Regulation | Regulated_by_gene | WBGene00014115 |
