WormBase Tree Display for Interaction: WBInteraction000558195
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| WBInteraction000558195 | Interaction_type | Regulatory | Change_of_expression_level | ||
|---|---|---|---|---|---|
| Interactor | Interactor_overlapping_gene | WBGene00000105 | Interactor_type | Trans_regulator | |
| WBGene00003026 | Interactor_type | Trans_regulated | |||
| Interaction_summary | "A lacZ-lin-41 fusion transgene expresses LacZ in the seam cells under the translational control of let-7 (ref. 19). In wild-type animals, LacZ staining in the seam cells can be observed from the L1 stage through to L4. The staining is absent in adult animals as a consequence of let-7-mediated repression (Fig. 4a, b). As expected, suppression of alg-1 and dcr-1 by RNAi resulted in persistence of LacZ staining in adults[20-22] (Fig. 4c, d). Next, we used RNAi to analyse the involvement of VIG-1 and TSN-1 in let-7 function. RNAi against either VIG-1 or TSN-1 results in persistent expression of the LacZ reporter in the seam cells of adult animals (Fig. 4e, f)." | ||||
| Detection_method | Reporter_gene | [lacZ::lin-41] | |||
| Regulation_result | Negative_regulate | Life_stage | WBls:0000041 | ||
| Anatomy_term | WBbt:0005753 | ||||
| Paper | WBPaper00006077 | ||||
| Remark | Fig. 4 |
