WormBase Tree Display for Interaction: WBInteraction000544061
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| WBInteraction000544061 | Interaction_type | Regulatory | Change_of_expression_level | ||
|---|---|---|---|---|---|
| Interactor | Interactor_overlapping_gene | WBGene00003096 | Interactor_type | Trans_regulated | |
| Other_interactor | Nypa fruticans root butanol extract | Interactor_type | Trans_regulator | ||
| Interaction_summary | "we tested the effect of these candidates on the expression of a host immune effector using transgenic worms carrying a transcriptional GFP reporter for the lys-7 gene... The transgenic animals were infected by S. aureus in the presence and absence of antiinfective candidates and fluorescence intensity was visualized and compared at 24 and 48 hours post-infection (hpi). As shown in Fig. 1A and Fig. 1B, at 48 hpi, nematodes infected by S. aureus in the absence of anti-infective candidates showed a significant reduction in the overall GFP intensity as compared to the uninfected population fed on the normal food source, Escherichia coli OP50. The uninfected worms showed a consistently strong green fluorescence along the intestine throughout the observation period (Fig. 1A) whereas S. aureus-infected worms exhibited weak fluorescence except at the posterior part of the intestine (Fig. 1B). Interestingly, among the anti-infective candidates tested, the UE-12 extract was able to prevent suppression of GFP expression by S. aureus (Fig. 1C). By contrast, UE-01-4, UE-03-6, UE-11 and UC-10 failed to prevent the suppression of GFP expression by S. aureus (supplementary material Fig. S1)." | ||||
| Detection_method | Reporter_gene | [lys-7p::gfp] | |||
| Regulation_level | Transcriptional | ||||
| Regulation_result | Does_not_regulate | Life_stage | WBls:0000041 | ||
| Anatomy_term | WBbt:0005772 | ||||
| Paper | WBPaper00045438 | ||||
| Remark | Fig. S1; Staphylococcus aureus infection |
