WormBase Tree Display for Interaction: WBInteraction000542323
expand all nodes | collapse all nodes | view schema
| WBInteraction000542323 | Interaction_type | Regulatory | Change_of_localization | ||
|---|---|---|---|---|---|
| Interactor | Interactor_overlapping_gene | WBGene00002275 | Interactor_type | Trans_regulated | |
| Transgene | WBTransgene00001856 | ||||
| Construct | WBCnstr00001838 | ||||
| WBGene00003210 | Interactor_type | Trans_regulator | |||
| Interaction_summary | After control RNAi treatment, GFP-LEM-2 was evenly distributed in pronuclear NEs, redistributed to the endoplasmic reticulum (ER) during mitosis, and recruited around chromosomes 60 to 100 s after anaphase entry. In mel-28(RNAi) one-cell embryos, no clear nuclear rim staining was visible despite the acquisition of several confocal sections at each time point. Small and misshapen pronuclei were sometime seen, and GFP signal concentrated around the centrosomes prior to mitosis. Later during mitosis, more GFP-LEM-2 accumulated at centrosomes than in control RNAi-treated embryos, but mitosis proceeded normally in terms of centrosome and cytokinesis. At mitotic exit, GFP-LEM-2 signal on the chromatin surface increased to a lesser degree than in the control RNAi embryos and at best formed a discontinuous, small, and misshapen NE-like structure. | ||||
| Detection_method | Construct | ||||
| Reporter_gene | [lem-2::gfp], translational fusion of GFP with LEM-1, driven by pie-1 promoter | ||||
| Transgene | |||||
| Regulation_result | Negative_regulate | Subcellular_localization | accumulated around centrosomes in mel-28(RNAi) embryos | ||
| Interaction_RNAi | WBRNAi00065562 | ||||
| Paper | WBPaper00028456 |
