WormBase Tree Display for Interaction: WBInteraction000542288

WBInteraction000542288 Interaction_type: Regulatory: Change_of_localization
  Interactor: Interactor_overlapping_gene: WBGene00004721 Interactor_type: Trans_regulated
        Expr_pattern: Expr2761
        Antibody: WBAntibody00000691
    Other_interactor: anoxia exposure Interactor_type: Trans_regulator
  Interaction_summary: 4-cell embryos exposed to anoxia contain detectable SAN-1 localized to the kinetochore and in lateral projections from the metaphase plate. There is less detectable SAN-1 in the nucleoplasm in metaphase blastomeres of embryos exposed to 30 minutes or 6 hours of anoxia in comparison to normoxic embryos. The lateral projections were not as easily detected in 8-cell embryos exposed to 6 hours or more of anoxia suggesting that either the kinetochore projections are only a characteristic of 4-cell embryos or the projections are more difficult to detect in embryos developed beyond the 4-cell stage. Embryos exposed to 12 hours or more of anoxia contains SAN-1 localized in punctate form in the nucleoplasm surrounding the metaphase plate. Embryos exposed to at least 24 hours of anoxia display a reduction in the detection of SAN-1 at the kinetochore. Taken together, it is concluded that SAN-1 localization changes are dependent upon anoxia exposure time.
  Detection_method: Antibody
  Regulation_result: Negative_regulate: Subcellular_localization: nucleus
  Paper: WBPaper00026995
  Remark: To gain a greater understanding of how anoxia affects SAN-1 sub-cellular localization, young embryos were exposed to anoxia for 30 minutes, 6, 12, 24 or 72 hours and stained with the DNA binding dye DAPI and an antibody to detect SAN-1. In all experiments, the antibody that detects the dimethylated Lysine 4 (MeH3) was used as an antibody accessibility control, because the localization of this antigen is not altered in mitotic blastomeres of embryos exposed to anoxia.