WormBase Tree Display for Interaction: WBInteraction000524957
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| WBInteraction000524957 | Interaction_type | Regulatory | Change_of_expression_level | ||
|---|---|---|---|---|---|
| Interactor | Interactor_overlapping_gene | WBGene00000898 | Interactor_type | Trans_regulator | |
| WBGene00006650 | Interactor_type | Trans_regulated | |||
| Variation_interactor | WBVar00143949 | Interactor_type | Trans_regulator | ||
| Interaction_summary | With the expression of pmp-3 set to 1, there is almost no detectable tts-1 expression in N2s or daf-2;daf-16 mutants. This is compared to an approximately 2-fold increase of expression of tts-1 over pmp-3 in the daf-2 mutants, confirming previous reports of high tts-1 expression in these mutants and also suggesting this expression is at least partly daf-16 dependent. Further qPCR results comparing the levels of tts-1 on N2 and daf-2 monosomes and polysomes reveal that compared to pmp-3, tts-1 is enriched over 12-fold in the daf-2 monosomal fraction and over 4-fold in the daf-2 polysomal fraction, with again almost no detectable tts-1 found in either fraction of the N2 strain. These data suggest that tts-1 is not only expressed at much higher levels in daf-2 mutants but also preferentially enriched on ribosomes. Fluorescent in situ hybridization analysis (FISH) with 11 different probes against tts-1 confirmed the substantially higher expression of tts-1 in daf-2 mutants compared to N2. | ||||
| Detection_method | In_situ | ||||
| RT_PCR | |||||
| Regulation_level | Transcriptional | ||||
| Paper | WBPaper00046337 |
