WormBase Tree Display for Interaction: WBInteraction000501565

WBInteraction000501565 Interaction_type: Regulatory
  Interactor: Interactor_overlapping_gene: WBGene00002299 Interactor_type: Trans_regulator
      WBGene00002992 Interactor_type: Trans_regulator
      WBGene00002996 Interactor_type: Trans_regulator
      WBGene00004400 Interactor_type: Trans_regulated
        Expr_pattern: Expr3457
    Variation_interactor: WBVar00143988 Interactor_type: Trans_regulator
  Interaction_summary: To test whether the inductive AC signal is required for the elevated rom-1::nls::gfp expression in the proximal VPCs, the precursors of the somatic gonad Z1 and Z4 in zhIs5 animals was ablated. Uniformly low rom-1::nls::gfp expression was found in all six VPCs of gonad-ablated zhIs5 animals at the late L2 to early L3 stage, before the descendants of the tertiary VPCs had fused to hyp7. To test whether rom-1::nls::gfp expression depends on RTK/RAS-/MAPK signaling in the VPCs, the zhIs5 transgene was introduced into lin-7(e1413) mutants that exhibit a penetrant Vul phenotype due to reduced LET-23 EGFR activity. In lin-7(e1413); zhIs5 animals, the up-regulation of rom-1::nls::gfp occurred less frequently (in 13%, 33%, and 7% of the cases in P5.p, P6.p, and P7.p, respectively, n = 15). Thus, the AC signal up-regulates rom-1::nls::gfp expression in the VPCs that adopt vulval cell fates.
  Detection_method: Reporter_gene: [rom-1::nls-gfp] translational fusion. A transcriptional rom-1 reporter was generated by fusing 6.9 kb of the 5 rom-1 promoter/enhancer region to the green fluorescent protein (gfp) ORF carrying a nuclear localizing signal (zhIs5[rom-1::nls::gfp]).
  Paper: WBPaper00024876