WormBase Tree Display for Interaction: WBInteraction000501470
expand all nodes | collapse all nodes | view schema
| WBInteraction000501470 | Interaction_type | Regulatory | |||
|---|---|---|---|---|---|
| Interactor | Interactor_overlapping_gene | WBGene00006791 | Interactor_type | Trans_regulator | |
| WBGene00006763 | Interactor_type | Trans_regulated | |||
| Expr_pattern | Expr2771 | ||||
| Variation_interactor | WBVar00091608 | Interactor_type | Trans_regulator | ||
| Interaction_summary | Synaptojanin-GFP appeared to be reduced in levels and was diffuse in unc-57(ok310) mutants, compared to the wild-type. Average synaptojanin-GFP fluorescence intensity was determined for the cell bodies and ventral cord in endophilin/unc-57 mutant and control worms. Fluorescence was reduced in unc-57(ok310) mutants by 16% in the cell bodies (average intensity on scale of 0 to 255: 98.5 in the control, 82.6 in unc-57(ok310), p=0.0006) and 25% in the ventral cord (average intensity is 70.5 in the control and 53.0 in unc-57(ok310), p=0.001). The loss of synaptojanin in axons was caused by a specific loss of accumulation at synapses. Peaks of fluorescence, corresponding to punctate localization of synaptojanin-GFP, were observed in control worms. unc-57(ok310) mutant worms did not exhibit peaks of fluorescence, but levels were constant along the length of the cord at the same levels as intersynaptic regions of the control. The loss of synaptojanin at synapses in unc-57 is not caused by a defect in synapse formation. | ||||
| Detection_method | Reporter_gene | [unc-26::gfp] translational fusion. | |||
| Regulation_result | Positive_regulate | Anatomy_term | WBbt:0005300 | ||
| Subcellular_localization | cell body and ventral cord. | ||||
| Paper | WBPaper00006213 |
