WormBase Tree Display for Gene: WBGene00003151
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WBGene00003151 | SMap | S_parent | Sequence | W09C2 | |||
---|---|---|---|---|---|---|---|
Identity (6) | |||||||
Gene_info | Biotype | SO:0001217 | |||||
Gene_class | mca | ||||||
Allele (95) | |||||||
Legacy_information | [Kraev A] Originally identified by similarity to mammalian PMCA family proteins in Genome Project data, mca-1 was expressed in insect cells and shown to have the predicted activity: aspartyl-phosphate formation in the presence of calcium, like mammalian counterparts, has a calmodulin-binding domain at the C-terminus and actually binds human calmodulin in a standard assay. Two other related sequences identified in the genomic sequencing data "in progress" and cDNAs finished by direct RT-PCR product sequencing. Mca-3 shows the highest sequence similarity to the mammalian analogues (77% on aminoacid). All three mca sequences have calmodulin-binding domains at the same location as their mammalian counterparts and the same predicted trans-membrane topology | ||||||
Strain | WBStrain00037024 | ||||||
RNASeq_FPKM (74) | |||||||
GO_annotation (25) | |||||||
Ortholog (40) | |||||||
Paralog (14) | |||||||
Structured_description | Concise_description | mca-1 encodes one of three C. elegans plasma membrane Ca2+ ATPases (PMCAs); by homology, MCA-1 is predicted to function as a molecular pump that couples ATP hydrolysis to extrusion of cytosolic Ca2+; when expressed in insect cells, MCA-1 exhibits calcium-pumping ATPase activity and binding to human calmodulin; large-scale RNAi screens indicate that mca-1 activity is required for normal coordinated locomotion and rates of growth; an mca-1::gfp reporter fusion is expressed solely in the excretory canal. | Paper_evidence | WBPaper00003407 | |||
WBPaper00029172 | |||||||
Curator_confirmed | WBPerson1843 | ||||||
Date_last_updated | 30 Apr 2007 00:00:00 | ||||||
Automated_description | Enables P-type calcium transporter activity and calmodulin binding activity. Involved in calcium ion transport and intracellular calcium ion homeostasis. Located in membrane. Expressed in excretory canal. | Paper_evidence | WBPaper00065943 | ||||
Curator_confirmed | WBPerson324 | ||||||
WBPerson37462 | |||||||
Inferred_automatically | This description was generated automatically by a script based on data from the WS291 version of WormBase | ||||||
Date_last_updated | 29 Nov 2023 00:00:00 | ||||||
Molecular_info | Corresponding_CDS | W09C2.3a | |||||
W09C2.3b | |||||||
W09C2.3c | |||||||
W09C2.3d | |||||||
Corresponding_CDS_history | W09C2.3:wp92 | ||||||
Corresponding_transcript | W09C2.3a.1 | ||||||
W09C2.3b.1 | |||||||
W09C2.3c.1 | |||||||
W09C2.3d.1 | |||||||
Other_sequence (51) | |||||||
Associated_feature (12) | |||||||
Experimental_info | RNAi_result | WBRNAi00088616 | Inferred_automatically | RNAi_primary | |||
WBRNAi00026522 | Inferred_automatically | RNAi_primary | |||||
WBRNAi00019742 | Inferred_automatically | RNAi_primary | |||||
WBRNAi00000551 | Inferred_automatically | RNAi_primary | |||||
WBRNAi00088723 | Inferred_automatically | RNAi_primary | |||||
WBRNAi00055018 | Inferred_automatically | RNAi_primary | |||||
WBRNAi00027503 | Inferred_automatically | RNAi_primary | |||||
WBRNAi00026523 | Inferred_automatically | RNAi_primary | |||||
Expr_pattern | Expr8318 | ||||||
Expr1025870 | |||||||
Expr1031487 | |||||||
Expr1158554 | |||||||
Expr2013442 | |||||||
Expr2031676 | |||||||
Drives_construct | WBCnstr00008393 | ||||||
WBCnstr00013258 | |||||||
WBCnstr00036215 | |||||||
Construct_product | WBCnstr00036215 | ||||||
Microarray_results (38) | |||||||
Expression_cluster (173) | |||||||
Interaction (72) | |||||||
Map_info | Map | IV | Position | 4.34306 | Error | 0.000339 | |
Positive | Positive_clone | W09C2 | Author_evidence | Kraev A | |||
Inferred_automatically | From sequence, transcript, pseudogene data | ||||||
Pseudo_map_position | |||||||
Reference | WBPaper00003407 | ||||||
WBPaper00011802 | |||||||
WBPaper00015567 | |||||||
WBPaper00038491 | |||||||
WBPaper00055090 | |||||||
Remark | Map position created from combination of previous interpolated map position (based on known location of sequence) and allele information. Therefore this is not a genetic map position based on recombination frequencies or genetic experiments. This was done on advice of the CGC. | CGC_data_submission | |||||
Method | Gene |