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WormBase Tree Display for Construct: WBCnstr00043075

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Name Class

WBCnstr00043075Public_namepRA642
Summaryhnd-1p::ZIF-1::Cre
Driven_by_geneWBGene00001981
GeneWBGene00006977
Construction_summaryThe hnd-1p::ZIF-1::Cre and hnd-1p::GFP-nanobody::ZIF-1::Cre repair plasmids were generated by digesting pRA638 with PacI and inserting sequences upstream of Cre. ZIF-1 and GFP-nanobody::ZIF-1 sequences were amplified from pOD2046 (Addgene #89367; Wang et al. 2017) using primers RA1506 and RA1507 (GFP-nanobody::ZIF-1) or RA1508 and RA1507 (ZIF-1). The operon linker in pOD2046 was retained to separate the GFP-nanobody::ZIF-1 (or ZIF-1) and Cre-coding sequences, creating bicistronic expression constructs. The products were cloned into pRA638 using the NEBuilder HiFi DNA Assembly Master Mix (NEB)
Used_forTransgene_constructWBTransgene00033743
ReferenceWBPaper00066277