WormBase Tree Display for Construct: WBCnstr00043075
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WBCnstr00043075 | Public_name | pRA642 | |
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Summary | hnd-1p::ZIF-1::Cre | ||
Driven_by_gene | WBGene00001981 | ||
Gene | WBGene00006977 | ||
Construction_summary | The hnd-1p::ZIF-1::Cre and hnd-1p::GFP-nanobody::ZIF-1::Cre repair plasmids were generated by digesting pRA638 with PacI and inserting sequences upstream of Cre. ZIF-1 and GFP-nanobody::ZIF-1 sequences were amplified from pOD2046 (Addgene #89367; Wang et al. 2017) using primers RA1506 and RA1507 (GFP-nanobody::ZIF-1) or RA1508 and RA1507 (ZIF-1). The operon linker in pOD2046 was retained to separate the GFP-nanobody::ZIF-1 (or ZIF-1) and Cre-coding sequences, creating bicistronic expression constructs. The products were cloned into pRA638 using the NEBuilder HiFi DNA Assembly Master Mix (NEB) | ||
Used_for | Transgene_construct | WBTransgene00033743 | |
Reference | WBPaper00066277 |