WormBase Tree Display for Construct: WBCnstr00042947
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| WBCnstr00042947 | Summary | [dnj-14p::human DNAJC5] | |
|---|---|---|---|
| Driven_by_gene | WBGene00001032 | ||
| Type_of_construct | Transcriptional_fusion | ||
| Construction_summary | A synthetic DNA construct of the human DNAJC5 coding sequence, codon-optimized for expression in C. elegans, was created by GeneArt (ThermoFisher, UK). The putative dnj-14 promoter was made by PCR cloning into the PD117.01 vector with the GFP coding sequence removed, as previously described (26). The synthetic DNAJC5 construct was then sub-cloned into this vector, creating a plasmid with human CSP expressed under control of the natural worm dnj-14 promoter. | ||
| Used_for | Transgene_construct | WBTransgene00033619 | |
| Reference | WBPaper00064620 | ||
| Laboratory | AMG |
