WormBase Tree Display for Construct: WBCnstr00038866
expand all nodes | collapse all nodes | view schema
| WBCnstr00038866 | Summary | [spin-3p::GFP::3xFlag::unc-54UTR] | |
|---|---|---|---|
| Driven_by_gene | WBGene00008598 | ||
| Fusion_reporter | mNeonGreen | ||
| Type_of_construct | Transcriptional_fusion | ||
| Construction_summary | [spin-3p::GFP::3xFlag::unc-54UTR] transcriptional fusion. The design of C. elegans-tailored mNeonGreen sequence implicated codon optimization and intron integration as previously described (Schild and Glauser 2015). The initial mNeonGreen plasmid (dg361) was obtained by gene synthesis (Genewiz). As starting plasmids for GFP constructs, we used the slot3 Gateway Entry vector pGH50 (gift from Erik Jorgensen). The 3xFlag tags were added by whole plasmid amplification with primer pairs, in which each primer contained half of the 3xFlag sequence, followed by ligation to produce dg432 and dg399. All other fluorescent protein constructions were made from these starting plasmids with standard restriction/ligation based cloning and/or recombination in the three-fragment Multisite Gateway system (Invitrogen). GFP transcriptional reporter generated by LR reaction between dg408, dg400, pMH473, and pCFJ150. | ||
| Used_for | Transgene_construct | WBTransgene00032067 | |
| Reference | WBPaper00050558 |
