WormBase Tree Display for Construct: WBCnstr00038209
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| WBCnstr00038209 | Public_name | pRS315-exc-6 | pJKL702-exc-6::gfp | |
|---|---|---|---|
| Summary | [EXC-6::GFP] | ||
| Driven_by_gene | WBGene00019030 | ||
| Gene | WBGene00019030 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | To produce an exc-6::gfp fusion, the coding sequence for GFP, together with four synthetic introns, was amplified by PCR from pPD95.75 (provided by A. Fire, Carnegie Institute of Washington, Washington DC), and a linker encoding Gly-Ala-Gly-Ala-Gly was added to itsc5' end before introduction immediately upstream of the exc-6 stop codon in pRS315-exc-6, again using the GeneCATCHR method. This exc-6::gfp sequence was introduced into the worm transformation vector pJKL702 (a gift from J.K. Liu, Cornell University, Ithaca, NY) by standard subcloning to generate pJKL702-exc-6::gfp. The genomic sequence of exc-6, including 5 kb upstream and 2 kb downstream, was obtained from the yeast artificial chromosome Y11F11 (provided by the Wellcome Trust Sanger Institute, Cambridge, UK) and subcloned into pRS315 [Sikorski and Hieter, 1989] using the Gene Cloning and Tagging of C. elegans Genes using Yeast Homologous Recombination (GeneCATCHR) method [Sassi et al., 2005] in the yeast strain ASHSY2 (a gift from A. Spence, University of Toronto, Toronto, Ontario), resulting in the plasmid pRS315-exc-6. | ||
| Used_for | Transgene_construct | WBTransgene00023607 | |
| WBTransgene00023720 | |||
| Reference | WBPaper00050308 |
