WormBase Tree Display for Construct: WBCnstr00020799
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| WBCnstr00020799 | Summary | [CYK-4(deletion C1)::GFP::pie-1 3'UTR] | |
|---|---|---|---|
| Driven_by_gene | WBGene00000875 | ||
| Gene | WBGene00000875 | ||
| UTR_3 | WBGene00004027 | ||
| Fusion_reporter | GFP | ||
| Construction_summary | ~2kb sequences upstream of cyk-4, cyk-4 genomic DNA tagged with C-terminal GFP coding sequences, and pie-1 3'UTR sequences were generated by overlapping PCR and inserted to pCFJ150 by SLiCE (Zhang et al., 2012). cyk-4 genomic sequences between BamHI and AvrII were recoded to generated RNAi resistant alleles. To introduce cyk-4 mutations, sequences covering mutations were generated by overlapping PCR using pCJF150-cyk-4-gfp as template and the primers MG4199/MG4070 and MG4200/MG4071 for deletion C1. Overlapping PCR products were inserted into pCFJ15-cyk-4-gfp linearized with NaeI by SLiCE. | ||
| Used_for | Transgene_construct | WBTransgene00021185 | |
| Reference | WBPaper00048425 |
