WormBase Tree Display for Construct: WBCnstr00019673
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| WBCnstr00019673 | Summary | [Pblmp-1::dgfp] | |
|---|---|---|---|
| Driven_by_gene | WBGene00003847 | ||
| Fusion_reporter | dGFP(destabilized GFP) | ||
| Type_of_construct | Transcriptional_fusion | ||
| Construction_summary | The Pblmp-1::dgfp fragment was generated by fusion PCR by fusing two PCR products corresponding to Pblmp-1 and the region containing dgfp and the unc-54 3' UTR. Pblmp-1 was PCR-amplified from genomic DNA using primers D24-5end5kb and d24Pgfp/r, and the region containing dgfp and unc-54 39UTR was PCRamplified from pPD95.75PEST (pYW807), which contains dgfp, using primers GFP/f and D. The two PCR products were then mixed and fused by fusion PCR using primers d24-5kbf-nest and D9. The Pblmp-1::blmp-1::gfp fragment was generated by fusion PCR by fusing two PCR products corresponding to blmp-1 genomic DNA and the region containing gfp and unc-54 3' UTR from the pPD95.75 plasmid. | ||
| Used_for | Transgene_construct | WBTransgene00031619 | |
| Interactor | WBInteraction000523864 | ||
| Reference | WBPaper00045431 |
