WormBase Tree Display for Construct: WBCnstr00019052
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| WBCnstr00019052 | Other_name | Expr11508_Ex | |
|---|---|---|---|
| Summary | [Psta-1 ::NLS::GFP] | ||
| Driven_by_gene | WBGene00013111 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Transcriptional_fusion | ||
| Construction_summary | [Psta-1 ::NLS::GFP] transcriptional fusion. To create Psta-1::GFP transgenic lines, the 5.6 kb intergenic region upstream of sta-1 plus sequences coding for the first 10 amino acid residues was amplified using Expand TM Long Template PCR System (Boehringer Mannheim, Mannheim, Germany) and subcloned inframe into the Bam HI/ Kpn I sites of pPD95.81 and pPD96.04 (kindly provided by A. Fire, Stanford, CA, USA). | ||
| Clone | pPD95.81 | ||
| pPD96.04 | |||
| Used_for | Transgene_construct | WBTransgene00031531 | |
| Reference | WBPaper00028315 |
