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WormBase Tree Display for Construct: WBCnstr00018249

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Name Class

WBCnstr00018249Summary[Pdlc-1::DLC-1::GFP; Crb-unc-119(+)]
Driven_by_geneWBGene00001005
GeneWBGene00001005
Fusion_reporterGFP
Construction_summaryThe plasmid containing Pdlc-1::dlc-1::gfp was generated by using standard Gateway techniques (Invitrogen, Naerum, Denmark). pENTRY clones for the dlc-1 promoter and open reading frame (ORF) were generated by using PCR products amplified with Gateway and gene-specific primers. The vector pJA256 (Addgene, Cambridge, MA, USA) was used as pENTRY clone for GFP. The Gateway destination vector was pCFJ150 (Addgene) containing an unc-119 rescue fragment.
Used_forTransgene_constructWBTransgene00018888
ReferenceWBPaper00044144