WormBase Tree Display for Construct: WBCnstr00018249
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WBCnstr00018249 | Summary | [Pdlc-1::DLC-1::GFP; Crb-unc-119(+)] | |
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Driven_by_gene | WBGene00001005 | ||
Gene | WBGene00001005 | ||
Fusion_reporter | GFP | ||
Construction_summary | The plasmid containing Pdlc-1::dlc-1::gfp was generated by using standard Gateway techniques (Invitrogen, Naerum, Denmark). pENTRY clones for the dlc-1 promoter and open reading frame (ORF) were generated by using PCR products amplified with Gateway and gene-specific primers. The vector pJA256 (Addgene, Cambridge, MA, USA) was used as pENTRY clone for GFP. The Gateway destination vector was pCFJ150 (Addgene) containing an unc-119 rescue fragment. | ||
Used_for | Transgene_construct | WBTransgene00018888 | |
Reference | WBPaper00044144 |