WormBase Tree Display for Construct: WBCnstr00017872
expand all nodes | collapse all nodes | view schema
WBCnstr00017872 | Other_name | Expr11043_Ex | |
---|---|---|---|
Summary | [pF48A11.1::GFP] | ||
Driven_by_gene | WBGene00000497 | ||
Fusion_reporter | GFP | ||
LacZ | |||
Type_of_construct | Transcriptional_fusion | ||
Construction_summary | The 5' region of the F48A11.1 gene (identified from C. elegans genomic sequences) was amplified using a proof-reading polymerase (Applied Biosystems) and cloned into the plasmid pPD96.04. The region from -1144 to +1 relative to the ATG start site was cloned into the Xba and BamHI-digested vector. The construct is a fusion of the chitin synthase 5' region, including the ATG, with the green fluorescent protein (GFP) and the E. coli -galactosidase (LacZ), and contains a nuclear localisation signal. | ||
Used_for | Transgene_construct | WBTransgene00018488 | |
Reference | WBPaper00004881 | ||
Remark | The 5' region of the F48A11.1 gene (identified from C. elegans genomic sequences) was amplified using a proof-reading polymerase (Applied Biosystems) and cloned into the plasmid pPD96.04 provided in the C.elegans vector kit, available from the laboratory of A.Fire (see ftp:ciw1ciwemb.edu). The region from -1144 to +1 relative to the ATG start site was cloned into the XbaI+ BamHI-digested vector. The construct is a translational fusion of the chitin synthase 5' region, including the ATG, with the green fluorescent protein (GFP) and the E. coli -galactosidase (LacZ), and contains a nuclear localisation signal. |