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WormBase Tree Display for Construct: WBCnstr00017872

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Name Class

WBCnstr00017872Other_nameExpr11043_Ex
Summary[pF48A11.1::GFP]
Driven_by_geneWBGene00000497
Fusion_reporterGFP
LacZ
Type_of_constructTranscriptional_fusion
Construction_summaryThe 5' region of the F48A11.1 gene (identified from C. elegans genomic sequences) was amplified using a proof-reading polymerase (Applied Biosystems) and cloned into the plasmid pPD96.04. The region from -1144 to +1 relative to the ATG start site was cloned into the Xba and BamHI-digested vector. The construct is a fusion of the chitin synthase 5' region, including the ATG, with the green fluorescent protein (GFP) and the E. coli -galactosidase (LacZ), and contains a nuclear localisation signal.
Used_forTransgene_constructWBTransgene00018488
ReferenceWBPaper00004881
RemarkThe 5' region of the F48A11.1 gene (identified from C. elegans genomic sequences) was amplified using a proof-reading polymerase (Applied Biosystems) and cloned into the plasmid pPD96.04 provided in the C.elegans vector kit, available from the laboratory of A.Fire (see ftp:ciw1ciwemb.edu). The region from -1144 to +1 relative to the ATG start site was cloned into the XbaI+ BamHI-digested vector. The construct is a translational fusion of the chitin synthase 5' region, including the ATG, with the green fluorescent protein (GFP) and the E. coli -galactosidase (LacZ), and contains a nuclear localisation signal.