WormBase Tree Display for Construct: WBCnstr00017410
expand all nodes | collapse all nodes | view schema
| WBCnstr00017410 | Other_name | Expr10632_Ex | |
|---|---|---|---|
| Summary | [Pect-2::ect-2-gfp::unc-119(+)] | ||
| Driven_by_gene | WBGene00002297 | ||
| Gene | WBGene00002297 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | GFP was amplified and inserted immediately prior to the stop codon of ect-2 within fosmid WRM0614aC02, using recombineering and galK replacement as described (Zhang et al., 2008b). The ect-2-gfp fusion, plus 2.2kb of upstream sequence and 3.8kb of downstream sequence, was subcloned into PCR-amplified unc-119 plasmid pPUB using recombineering (Sarov et al., 2006). --Precise ends. | ||
| Used_for | Transgene_construct | WBTransgene00019296 | |
| Reference | WBPaper00042051 |
