WormBase Tree Display for Construct: WBCnstr00015495
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WBCnstr00015495 | Other_name | Expr10150_Ex | |
---|---|---|---|
Summary | [Ce-age-1p::egfp::Ce-tbb-2t] | ||
Driven_by_gene | WBGene00000090 | ||
Gene | WBGene00006537 | ||
Fusion_reporter | GFP | ||
Construction_summary | The construct Ce-age-1p::egfp::Ce-tbb-2t, contained in plasmid pPV452, was assembled in two steps. The 866 bp of Ce-age-1 5' region was PCR amplified using C. elegans genomic DNA as a template. The 870 bp egpf coding region, including introns, and 333 bp of the Ce-tbb-2 3' region (terminator) were amplified using pJA257 as template (Addgene, Cambridge, Massachusetts, USA). These PCR products were fused by overlap extension PCR using the overlapping primers Ce-age-1-EGFP-F (caagctttcattttaagattttaagATGAGTAAAGGAGAAGAACTTTTC) and -R (GAAAAGTTCTTCTCCTTTACTCATcttaaaatcttaaaatgaaagcttg). The product was cloned into pUC19 (New England Biolabs) and the insert fully sequenced. | ||
Used_for | Transgene_construct | WBTransgene00015926 | |
Reference | WBPaper00041188 |