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WormBase Tree Display for Construct: WBCnstr00015495

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Name Class

WBCnstr00015495Other_nameExpr10150_Ex
Summary[Ce-age-1p::egfp::Ce-tbb-2t]
Driven_by_geneWBGene00000090
GeneWBGene00006537
Fusion_reporterGFP
Construction_summaryThe construct Ce-age-1p::egfp::Ce-tbb-2t, contained in plasmid pPV452, was assembled in two steps. The 866 bp of Ce-age-1 5' region was PCR amplified using C. elegans genomic DNA as a template. The 870 bp egpf coding region, including introns, and 333 bp of the Ce-tbb-2 3' region (terminator) were amplified using pJA257 as template (Addgene, Cambridge, Massachusetts, USA). These PCR products were fused by overlap extension PCR using the overlapping primers Ce-age-1-EGFP-F (caagctttcattttaagattttaagATGAGTAAAGGAGAAGAACTTTTC) and -R (GAAAAGTTCTTCTCCTTTACTCATcttaaaatcttaaaatgaaagcttg). The product was cloned into pUC19 (New England Biolabs) and the insert fully sequenced.
Used_forTransgene_constructWBTransgene00015926
ReferenceWBPaper00041188