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WormBase Tree Display for Construct: WBCnstr00015104

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Name Class

WBCnstr00015104Summary[pdk-1(KD)]
Driven_by_geneWBGene00003965
GeneWBGene00003965
UTR_3WBGene00003965
Construction_summaryA 9.2-kb PCR product of genomic DNA from the pdk-1(+) genomic region containing 2.7 kb of 5' upstream regulatory sequence, 6.1 kb of coding sequencing containing introns and exons,and 0.4 kb of pdk-1 3' UTR was purified using QIAquick (Qiagen) and injected with ttx-3::GFP (pPD95.75-C40H5-GFP O. Hobert, MGH and Harvard Medical School) as a co-injection marker (Mello et al. 1991). pdk-1(KD)was constructed and injected in the same manner except PCR primers were used to introduce the K98N mutation.
Used_forTransgene_constructWBTransgene00015516
ReferenceWBPaper00003568