WormBase Tree Display for Construct: WBCnstr00015104
expand all nodes | collapse all nodes | view schema
WBCnstr00015104 | Summary | [pdk-1(KD)] | |
---|---|---|---|
Driven_by_gene | WBGene00003965 | ||
Gene | WBGene00003965 | ||
UTR_3 | WBGene00003965 | ||
Construction_summary | A 9.2-kb PCR product of genomic DNA from the pdk-1(+) genomic region containing 2.7 kb of 5' upstream regulatory sequence, 6.1 kb of coding sequencing containing introns and exons,and 0.4 kb of pdk-1 3' UTR was purified using QIAquick (Qiagen) and injected with ttx-3::GFP (pPD95.75-C40H5-GFP O. Hobert, MGH and Harvard Medical School) as a co-injection marker (Mello et al. 1991). pdk-1(KD)was constructed and injected in the same manner except PCR primers were used to introduce the K98N mutation. | ||
Used_for | Transgene_construct | WBTransgene00015516 | |
Reference | WBPaper00003568 |