WormBase Tree Display for Construct: WBCnstr00013739
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| WBCnstr00013739 | Other_name | Expr9135_Ex | |
|---|---|---|---|
| Summary | [Ppxn-2-YFP::PXN-2] | ||
| Driven_by_gene | WBGene00004257 | ||
| Gene | WBGene00004257 | ||
| Fusion_reporter | YFP | ||
| RFP | |||
| Venus | |||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [Ppxn-2-YFP::PXN-2] translational fusion. To generate YFP-tagged PXN-2 authors used Gateway cloning (Invitrogen) to insert a PXN-2 cDNA lacking its N-terminal signal sequence into the vector pCZGY20, which contains the KAL-1 secretion signal sequence followed by Venus YFP (Hudson et al., 2006). Authors then inserted the 1.8 kb pxn-2 promoter to create Ppxn-2-YFP::PXN-2 (pCZGY926). pCZGY926 was injected at 50 ng/ul with the Pttx-3-RFP marker to generate arrays juEx2492-2495; images in Fig. 3 are of juEx2492. Primer AC1748: AATAAAGCGGCCGCTCATTTGCAGTCAAGCAGTGG. Primer AC1749: AACCAGCACATCTGCATTCC. --precise ends. | ||
| Used_for | Transgene_construct | WBTransgene00030924 | |
| Reference | WBPaper00037647 |
