WormBase Tree Display for Construct: WBCnstr00013723
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| WBCnstr00013723 | Other_name | Expr9104_Ex | |
|---|---|---|---|
| Summary | [ctn-1p::ctn-1-gfp] | ||
| Driven_by_gene | WBGene00000832 | ||
| Gene | WBGene00000832 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [ctn-1p::ctn-1-gfp] translational fusion constructs. To determine the expression patterns of different splicing variants of ctn-1, authors cloned ctn-1 genomic DNA including 4.3 kb upstream of the initiation site and 0.6 kb downstream of the last exon, and inserted GFP into a unique axon of each splicing variant. As the genomic DNA of ctn-1 was too long (B16 kb) to be cloned into one plasmid, it was cloned into two plasmids as two separate fragments with 0.6 kb overlap. Homologous recombination in vivo would result in a full-length CTN-1::GFP translational fusion. After linearization, the plasmids were coinjected with a lin-15 rescue plasmid into the lin-15(n765) strain. | ||
| Used_for | Transgene_construct | WBTransgene00030908 | |
| Reference | WBPaper00036762 |
