WormBase Tree Display for Construct: WBCnstr00013464
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| WBCnstr00013464 | Other_name | Expr8695_Ex | |
|---|---|---|---|
| Summary | [lpin-1::gfp-lpin-1] | ||
| Driven_by_gene | WBGene00010425 | ||
| Gene | WBGene00010425 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [lpin-1::gfp-lpin-1] translational fusion. The lpin-1 promoter (~1.9 kb upstream of the ATG) was PCR amplified with primers B4 LPN F1 (GGGGACAACTTTGTATAGAAAAGTTGaagctgtaatatcaacctgcg) and GFP LPN R1 (GAAAAGTTCTTCTCCTTTACTCATttgcgtccctgtgccaattaa) and GFP sequences were PCR amplified with primers LPN GFP F1 (ttaattggcacagggacgcaaATGAGTAAAGGAGAAGAACTTTTC) and B1 GFP R1 (GGGGACTGCTTTTTTGTACAAACTTGGtttgtatagttcatccatgccatg). These two PCR products were annealed and amplified with the outside primers to generate a lpin-1 promoter fused to GFP. --precise ends. | ||
| Clone | R1 | ||
| Used_for | Transgene_construct | WBTransgene00030657 | |
| Reference | WBPaper00033438 |
