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WormBase Tree Display for Construct: WBCnstr00011882

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Name Class

WBCnstr00011882Other_nameExpr4283_Ex
Summary[tbx-2::gfp]
Driven_by_geneWBGene00006543
GeneWBGene00006543
Fusion_reporterGFP
Type_of_constructTranslational_fusion
Construction_summary[tbx-2::gfp] translational fusion. A tbx-2::GFP translational fusion that included 5.2 kb upstream sequences with GFP at the carboxyl terminus. The 5.2 kb promoter tbx-2::GFP transgenic strain was produced by PCR amplification with forward primer 5'-GATTTATTCTCGTGCCTCGGTTCTCCTTTGAAG-3' and reverse primer 5'-AGGCTTTGACAACAAATCGAGAACATCAAAACC-3' (penultimate codon from Y54H5). The product was re-amplified with nested primers 5'-TTAAGAAGCATTTGAAAGCA-3' and 5'-AGTCGACCTGCAGGCATGCAAGCTAGGCTTTGACAACAAATCGAG-3', which placed GFP at the 3' end of the tbx-2 coding region. Independently, the entire GFP and unc-54 terminator sequence of pPD95.75 was amplified using forward primer 5'-AGCTTGCATGCCTGCAGGTCGACT-3' and reverse primer 5'-AAGGGCCCGTACGGCCGACTAGTAGG-3'. The tbx-2 and pPD95.75 PCR products were used to generate the 5.2 kb tbx-2::GFP-unc-54 reporter DNA. --precise ends.
ClonepPD95.75
Used_forTransgene_constructWBTransgene00029095
ReferenceWBPaper00028546