WormBase Tree Display for Construct: WBCnstr00011882
expand all nodes | collapse all nodes | view schema
WBCnstr00011882 | Other_name | Expr4283_Ex | |
---|---|---|---|
Summary | [tbx-2::gfp] | ||
Driven_by_gene | WBGene00006543 | ||
Gene | WBGene00006543 | ||
Fusion_reporter | GFP | ||
Type_of_construct | Translational_fusion | ||
Construction_summary | [tbx-2::gfp] translational fusion. A tbx-2::GFP translational fusion that included 5.2 kb upstream sequences with GFP at the carboxyl terminus. The 5.2 kb promoter tbx-2::GFP transgenic strain was produced by PCR amplification with forward primer 5'-GATTTATTCTCGTGCCTCGGTTCTCCTTTGAAG-3' and reverse primer 5'-AGGCTTTGACAACAAATCGAGAACATCAAAACC-3' (penultimate codon from Y54H5). The product was re-amplified with nested primers 5'-TTAAGAAGCATTTGAAAGCA-3' and 5'-AGTCGACCTGCAGGCATGCAAGCTAGGCTTTGACAACAAATCGAG-3', which placed GFP at the 3' end of the tbx-2 coding region. Independently, the entire GFP and unc-54 terminator sequence of pPD95.75 was amplified using forward primer 5'-AGCTTGCATGCCTGCAGGTCGACT-3' and reverse primer 5'-AAGGGCCCGTACGGCCGACTAGTAGG-3'. The tbx-2 and pPD95.75 PCR products were used to generate the 5.2 kb tbx-2::GFP-unc-54 reporter DNA. --precise ends. | ||
Clone | pPD95.75 | ||
Used_for | Transgene_construct | WBTransgene00029095 | |
Reference | WBPaper00028546 |