WormBase Tree Display for Construct: WBCnstr00011579
expand all nodes | collapse all nodes | view schema
| WBCnstr00011579 | Other_name | Expr3659_Ex | |
|---|---|---|---|
| Summary | [rbg-3::gfp] | ||
| Driven_by_gene | WBGene00007167 | ||
| Gene | WBGene00007167 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [rbg-3::gfp] translational fusion. For the rbg-3::gfp constuct, the forward primer (A) 5#-CGTCACCAACTACGGTATGTGTGG-3# and the reverse primer (B) 5#-AGTCGACCTGCAGGCATGCAAGCTCAATGGAGGGCGTTTTGTTGCC-3# was used to amplify the 1.9 kb promoter and 2.5 kb coding region. For nested 5# (A*), primer 5#-CGAGAAATGCGGAGAAATTGCG-3# was used. The resulting PCR products were purified using PCR purification kit (Qiagen) and injected at 50 ng/ul into the gonad of worms along with pRF4, (rol-6(su1066) plasmid) as a coinjection marker (100 ng/ul) to generate stable extrachromosomal transgenic lines. --precise ends. | ||
| Clone | pRF4 | ||
| Used_for | Transgene_construct | WBTransgene00028796 | |
| Reference | WBPaper00026699 |
