WormBase Tree Display for Construct: WBCnstr00011199
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WBCnstr00011199 | Other_name | Expr3179_Ex | |
---|---|---|---|
Summary | [K10D6.2a::gfp] | ||
Driven_by_gene | WBGene00010742 | ||
Gene | WBGene00010742 | ||
Fusion_reporter | GFP | ||
Type_of_construct | Translational_fusion | ||
Construction_summary | [K10D6.2a::gfp] translational fusion. A genomic DNA fragment was amplified by PCR containing a 3.45kb upstream promoter region followed by 265aa of predicted coding region. The 3' PCR primer is CTTCGATATCCTGCTGACGACGATCTTCG. The 5' primer is GATTAGCTCGAGTTGCGGTTAGT. The amplified PCR fragment was digested by XhoI and EcoRV, then cloned in-frame into pPD95.75 to create GFP fusion construct. --precise ends. | ||
Clone | pPD95.75 | ||
Used_for | Transgene_construct | WBTransgene00028419 | |
Reference | WBPaper00024994 |