WormBase Tree Display for Construct: WBCnstr00011166
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| WBCnstr00011166 | Other_name | Expr3138_Ex | |
|---|---|---|---|
| Summary | [dpy-31::nls-gfp] | ||
| Driven_by_gene | WBGene00006592 | ||
| Gene | WBGene00006592 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [dpy-31::nls-gfp] and [dpy-31::gfp] translational fusion constrcuts. A 3.4-kb PCR product was amplified from cosmid R151 using the following primers: PstIPrF sense (primer used for construct pD31FL) and BaPrR antisense (BamHI site is underlined): 5'-cgggatcccgGCTGAAATTAAAGTTTAAAG-3'. The resulting PCR product was digested and cloned directionally into pPD96.04. pD31P3.4G-N, a version of pD31P3.4G devoid of nuclear localization signal (NLS), was generated by digestion of pD31P3.4G with KpnI followed by religation. --precise ends. | ||
| Clone | R151 | ||
| Used_for | Transgene_construct | WBTransgene00028386 | |
| Reference | WBPaper00024637 |
