WormBase Tree Display for Construct: WBCnstr00011074
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| WBCnstr00011074 | Other_name | Expr3002_Ex | |
|---|---|---|---|
| Summary | [gar-3::gfp] | ||
| Driven_by_gene | WBGene00001519 | ||
| Fusion_reporter | GFP | ||
| Construction_summary | [gar-3::gfp]. The gar-3::GFP expression construct was generated by overlap-extension PCR using wild-type genomic DNA and vector pPD95.77 as templates. The 5' forward primer is located ~6000 bp upstream of the gar-3 ATG. Primers used were: gar-3Pro, 5'-ggt aca cag ctc ctt tcc aat caa-3'; gfp-gar-3F, 5'-cag aca cac tta ttc cag atg ttg gcc aaa gga ccc aaa g-3'; gfp-gar-3R, 5'-ctt tgg gtc ctt tgg cca aca tct gga ata agt gtg tct g-3'; and gfp-R, 5'-caa acc caa acc ttc ttc cga tc-3'. --precise ends. | ||
| Clone | pPD95.77 | ||
| Used_for | Transgene_construct | WBTransgene00028300 | |
| Reference | WBPaper00024250 |
